Coding

Part:BBa_J120000:Design

Designed by: Corinthia Black, Sheree Harper, Srikanth Sistla, Anna Wieman   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-10)


Coding sequence for PARP1 (poly [ADP-ribose] polymerase 1) H. sapiens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1599
    Illegal BamHI site found at 2361
    Illegal BamHI site found at 2612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2700
    Illegal BsaI.rc site found at 2738


Design Notes

Two internal restriction enzyme recognition sites were found in the sequence. To prevent cleavage by restriction enzymes, the third base of the codons at these sites were changed.


Source

NCBI (accession # NM_001618.3)

References